2007-07-20 11:36:26 +02:00
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import sys
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import rpy
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from pylab import gca, figure, subplot
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from scipy import *
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from lpls import *
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import rpy_go
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sys.path.append("../../fluents") # home of dataset
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sys.path.append("../../fluents/lib") # home of cx_stats
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import dataset
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import cx_stats
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from plots_lpls import plot_corrloads
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######## DATA ##########
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# full smoker data
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DX = dataset.read_ftsv(open("../../data/smokers-full/Xfull.ftsv"))
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DY = dataset.read_ftsv(open("../../data/smokers-full/Yg.ftsv"))
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Y = DY.asarray()
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# select subset genes by SAM
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rpy.r.library("siggenes")
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rpy.r.library("qvalue")
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data = DX.asarray().T
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# data = data[:100,:]
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rpy.r.assign("data", data)
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cl = dot(DY.asarray(), diag([1,2,3])).sum(1)
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rpy.r.assign("cl", cl)
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rpy.r.assign("B", 100)
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# Perform a SAM analysis.
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print "Starting SAM"
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sam = rpy.r('sam.out<-sam(data=data,cl=cl,B=B,rand=123)')
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print "SAM done"
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# Compute the q-values of the genes.
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qq = rpy.r('qobj<-qvalue(sam.out@p.value)')
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qvals = asarray(qq['qvalues'])
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# cut off
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2007-07-20 14:32:54 +02:00
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co = 0.1
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2007-07-20 11:36:26 +02:00
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index = where(qvals<0.01)[0]
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# Subset data
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X = DX.asarray()
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Xr = X[:,index]
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gene_ids = DX.get_identifiers('gene_ids', index)
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### Build GO data ####
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print "Go terms ..."
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goterms = rpy_go.goterms_from_gene(gene_ids)
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terms = set()
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for t in goterms.values():
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terms.update(t)
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terms = list(terms)
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rpy.r.library("GOSim")
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# Go-term similarity matrix
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methods = ("JiangConrath","Resnik","Lin","CoutoEnriched","CoutoJiangConrath","CoutoResnik","CoutoLin")
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2007-07-20 14:32:54 +02:00
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meth = methods[2]
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2007-07-20 11:36:26 +02:00
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print "Term-term similarity matrix (method = %s)" %meth
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if meth=="CoutoEnriched":
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rpy.r('setEnrichmentFactors(alpha=0.1,beta=0.5)')
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tmat = rpy.r.getTermSim(terms, verbose=False, method=meth)
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# check if all terms where found
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nanindex = where(isnan(tmat[:,0]))[0]
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keep=[]
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has_miss = False
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if len(nanindex)>0:
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has_miss = True
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print "Some terms missing in similarity matrix"
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keep = where(isnan(tmat[:,0])!=True)[0]
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print "Number of nans: %d" %len(nanindex)
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tmat_new = tmat[:,keep][keep,:]
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new_terms = [i for ind,i in enumerate(terms) if ind in keep]
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bad_terms = [i for ind,i in enumerate(terms) if ind not in keep]
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# update go-term dict
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for gene,trm in goterms.items():
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for t in trm:
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if t in bad_terms:
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trm.remove(t)
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if len(trm)==0:
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print "Removing gene: %s" %gene
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goterms[gene]=trm
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terms = new_terms
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tmat = tmat_new
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# Z-matrix
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# func (min, max, median, mean, etc),
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# func decides on the representation of gene-> goterm when multiple
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# goterms exist for one gene
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Z, newind = rpy_go.genego_matrix(goterms, tmat, gene_ids, terms,func=mean)
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Z = Z.T
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# update X matrix (no go-terms available)
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Xr = Xr[:,newind]
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gene_ids = asarray(gene_ids)[newind]
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######## LPLSR ########
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print "LPLSR ..."
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a_max = 5
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aopt = 2
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alpha=.5
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T, W, P, Q, U, L, K, B, b0, evx, evy, evz = nipals_lpls(Xr,Y,Z, a_max, alpha)
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# Correlation loadings
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dx,Rx,ssx= correlation_loadings(Xr, T, P)
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dx,Ry,ssx= correlation_loadings(Y, T, Q)
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cadx,Rz,ssx= correlation_loadings(Z.T, K, L)
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# Prediction error
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rmsep , yhat, class_error = cv_lpls(Xr, Y, Z, a_max, alpha=alpha)
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# Significance Hotellings T
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Wx, Wz, Wy, = jk_lpls(Xr, Y, Z, aopt)
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tsqx = cx_stats.hotelling(Wx,W[:,:aopt])
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tsqz = cx_stats.hotelling(Wz,L[:,:aopt])
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## plots ##
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figure(1) #rmsep
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2007-07-20 14:32:54 +02:00
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bar_w = .2
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bar_col = 'rgb'*5
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m = Y.shape[1]
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for a in range(m):
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bar(arange(a_max)+a*bar_w+.1, rmsep[:,a], width=bar_w, color=bar_col[a])
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ylim([rmsep.min()-.05, rmsep.max()+.05])
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2007-07-20 11:36:26 +02:00
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figure(2) # Hypoid correlations
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2007-07-20 14:32:54 +02:00
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title('RMSEP')
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2007-07-20 11:36:26 +02:00
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plot_corrloads(Rz, pc1=0, pc2=1, s=tsqz/10.0, c='b', zorder=5, expvar=evz, ax=None)
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ax = gca()
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2007-07-20 14:32:54 +02:00
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ylabels = DY.get_identifiers('_cat', sorted=True)
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plot_corrloads(Ry, pc1=0, pc2=1, s=150, c='g', zorder=5, expvar=evy, ax=ax,labels=ylabels)
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2007-07-20 11:36:26 +02:00
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figure(3)
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subplot(221)
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ax = gca()
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plot_corrloads(Rx, pc1=0, pc2=1, s=tsqx/2.0, c='b', zorder=5, expvar=evx, ax=ax)
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# title('X correlation')
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subplot(222)
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ax = gca()
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plot_corrloads(Ry, pc1=0, pc2=1, s=150, c='g', zorder=5, expvar=evy, ax=ax)
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#title('Y correlation')
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subplot(223)
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ax = gca()
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plot_corrloads(Rz, pc1=0, pc2=1, s=tsqz/10.0, c='r', zorder=5, expvar=evz, ax=ax)
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#title('Z correlation')
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subplot(224)
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plot(arange(len(evx)), evx, 'b', label='X', linewidth=2)
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plot(evy, 'g', label='Y', linewidth=2)
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plot(evz, 'r', label='Z', linewidth=2)
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legend(loc=2)
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ylabel('Explained variance')
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xlabel('Component')
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show()
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